The process of pre treating impurities in fermentation liquid using a three effect evaporator

   1 Centrifuge

Centrifugation is a common solid-liquid separation method that can effectively remove large molecular substances such as bacteria and proteins. However, due to the high rotational speed required and the small sample processing capacity, equipment investment and energy consumption are high, making it unsuitable for industrial production.

2 membrane filtration

This method uses filter media with certain selective permeability to separate target substances, commonly including microfiltration, ultrafiltration, and nanofiltration, which can be separated according to the different molecular weights of each substance. The pretreatment of fermentation broth can generally use ultrafiltration membrane filtration to remove large molecular substances from the fermentation broth.

3 yeast liquid triple effect evaporator for evaporation and concentration

The function of evaporation concentration is to remove excess water from the fermentation broth during the fermentation process. By using a double effect falling film evaporator or a triple effect falling film evaporator, the water in the material can be quickly evaporated without damaging its activity. The equipment has the following characteristics: high equipment height, utilizing the principle of pump and gravity, material flash evaporation from top to bottom, achieving the purpose of evaporation. 2. The equipment design is compact and occupies a small area. 3. Continuous material feeding can save excess labor costs.

4 flocculation

Flocculation is the process of adding coagulants to aggregate suspended particles in a liquid that are not easily settled into clusters, making them easier to settle.

5. Two phase aqueous extraction

When hydrophilic organic solvents (small molecule alcohols and ketones) are mixed with water and inorganic salts in appropriate proportions, liquid-liquid stratification occurs, and this biphasic system is called a novel biphasic system. In this new type of aqueous two-phase system, PDO and other alcohol substances (such as residual glycerol) in the fermentation broth mainly enter the organic phase (extraction phase), while other components in the fermentation broth mainly enter the aqueous phase (extraction phase), which can remove the vast majority of bacterial cells, proteins, salts, and other impurities in the fermentation broth.